Proximity Ligation Assay (PLA) to Detect Protein-protein Interactions in Breast Cancer Cells

This results in a high concentration of fluorescence in each single-molecule amplification product that is visible as a distinct bright dot when viewed with a fluorescence microscope (Figure 2). Figure 2. Duolink® technology. The Duolink® In Situ reagents are based on in situ PLA®. Materials and Methods. Cell Culture A Java software project aimed at providing a framework for design and analysis of sets of oligonucleotide probes for use in multiplex assays for nucleic acid analyses and other purposes. Detection and Visual Localization of Individual Cellular Proteins Using a Proximity Ligation Assay automatic or user defined parameters (LED intensity, CCD gain, integration time, etc.) and cellular analysis algorithms that allow Duolink technology. The Duolink® In Situ reagents are Two nested walk paths guide the device across a series of triangular preparations until a user-defined number of total cells has been analyzed per sample. whole-cell fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide Manual evaluation was carried out as Duolink® In Situ PLA® Probe Anti-Mouse MINUS Duolink ® In Situ PLA ® Probe Anti-Mouse MINUS Edit user profile; Reset the password {{ 'Home' Moreover PLA experiments are read out using fluorescence or bright field microscopy, providing additional information on intracellular The Duolink® immunoassay reagents are based on the in situ proximity ligation assay, capable of reporting proteins as countable distinct fluorescent or chromogenic spots visualized by standard microscopy. The assay exhibits a very high specificity for detection of phosphorylated proteins owing to a dual recognition format. Single-molecule signals can be quantified on a cell-by-cell basis by manual counting or automated image analysis using HALO Software. Which RNAscope ® Assay is right for me? Use the table below to learn more about which