Effectene Transfection Reagent Handbook: www.qiagen.com/HB-2084 This protocol is for transient or stable transfection of adherent cells in 6-well plates. For. 1 May 2013 In this protocol, S2 cells are transfected with dsRNA using QIAGEN's Effectene reagent, which has proven to be reliable for many investigators.Effectene Transfection Reagent is a nonliposomal lipid reagent for DNA transfection into a broad range of cell types. Due to its low cytotoxicity, Effectene QIAGEN. Highly efficient stable and transient transfection using Effectene® Reagent. Catherine the Effectene Transfection Reagent Handbook. Plasmids were REAGENTS. Many reagents are now available for optimization of cell transfection efficiency. References: Effectene Transfection Reagent Handbook (Qiagen). 1 May 2002 Effectene Transfection Reagent Handbook 05/2002. 4. Primary Cells and Cell Lines. 21. Adherent cells. 21. Suspension cells. 22. Primary cell 8 Nov 2006 A review of the Effectene™ Transfection Reagent From Qiagen . The Qiagen handbook has a variety of different optimization tips and (EN) - Effectene Transfection Reagent Handbook. Print Bookmark Share. more.. Back. Contact QIAGEN · Global contacts · Technical Service · Customer Care. In the 'gelatin-DNA' method the lipid-based transfection reagent is added to all the that Effectene and Lipofectamine 2000 work reasonably well with this protocol. Effectene Transfection Reagent (Qiagen catalog #301425); EC buffer (from Effectene Transfection Reagent. 10. mRNA Transfection. 12. TransMessenger Transfection Reagent. 12. Transfection Resources. 13. TransFect Protocol