Additionally, though the FDA Bacteriological Analytical Manual (FDA BAM) includes a real-time PCR screening method, the results must be confirmed culturally . The recommended cultural methods can also be used independently for identification of suspect isolates when PCR-based methods are not available . FDA/CFSAN - BAM * Appendix 1 - Rapid Methods for Detecting Foodborne Pathogens Page 2 of 14 new methods are often referred to as "rapid methods", a subjective term used loosely to describe a vast array of tests that includes miniaturized biochemical kits, antibody- and DNA-based tests, and Approximate pH of Selected Foods Examination of canned foods. FDA Bacteriological Analytical Manual, 8th Ed. Chapter 21, Table 11, AOAC International, Gaithersburg, MD 20877 4. Grahn M.A. 1984. Acidified and low acid foods from Southeast Asia. FDA-LIB FDA's Bacteriological Analytical Manual (The BAM) is a collection of procedures preferred by analysts in U.S. Food and Drug Administration laboratories for the detection in food and cosmetic products of pathogens (bacterial, viral, parasitic, plus yeast and mold) and of microbial toxins. Note: Citations are based on reference standards. However, formatting rules can vary widely between applications and fields of interest or study. The specific requirements or preferences of your reviewing publisher, classroom teacher, institution or organization should be applied. BAM FDA Bacteriological Analytical Manual CBI Confidential Business Information CCB Continuing Calibration Blank CCV Continuing Calibration Verifications cGMP Current Good Manufacturing Practices COC Chain-of-Custody DAD Diode Array Detection DOC Demonstration of Capability DOT Department of Transportation DQI Data Quality Indicators procedure outlined in FDA Bacteriological Analytical Manual, 8th Edition. January 2003, Chapter 10 Note: Roast Beef Aerobic Plate Count = 1.0 X 10^4 cfu/g. Analyze aerobic plate count (APC) and E. coli/coliform A total of 201 sample